Phytochemical analysis and anticancer activity of leaf extract of Mangifera indica (kotttukonam varika).
Helen P. A. M., Aswathy M. R., Rathi K. G. D. R. M., Joseph J. J., Sree S. J.
Author Affiliation: Department of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakkavilai - 629 153, Kanyakumari District, Tamil Nadu, India.
International Journal of Pharmaceutical Sciences and Research (IJPSR) 4 : 823-828
Abstract : About 6 extract (methanol, acetone, hexane, ethyl acetate, hexane-ethyl acetate, aqueous extraction) from the leaves of Mangifera indica was extracted. Hexane-ethyl acetate was characterized by Gas Chromatography-Mass Spectroscopy. Ten constituents from 10 peaks were identified. Terpinyl acetate (5.80%) and phytol isomer (5.12%) are as the major constituents and the minor constituents like oxirane (3.57%), sabinene (3.24%), beta-pinen (3.34%), beta-myrcene (3.23%), cymene (3.68%), alpha-limonene (2.82%), eucalyptol (1,8-cineo (4.71%)), 1,3-benzodioxole, 5-(2-, (3.68%)) were identified. The antimicrobial activity of different extract was tested against human and plant pathogenic bacteria. Hexane-ethyl acetate extract showed significant role on inhibiting almost all tested pathogenic organisms. Using histochemical reagents such as Phloroglucinol-HCl, Dragendorff reagent was used for the localization of lignin and alkaloids respectively. Mangifera indica showed 100% larvicide activity. The addition of various concentrations of hexane-ethyl acetate extract of Mangifera indica on the L929 cell lines showed cytotoxic activity.