Isolation and characterization of phosphate solubilising bacteria from rhizospheric soil samples.
Omkar Shankarrao
Author Affiliation: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431 606, Maharashtra, India.
Online International Interdisciplinary Research Journal 2 : 28-29
Abstract : Most of the soil phosphate found in insoluble form making unavailable to plant. To circumvent the phosphate deficiency, phosphate solubilizing microorganisms play an important role in supplying P to plants. In the present study total 28 Phosphate Solubilising Bacteria were isolated from rhizospheric soil of Neem, Mango and Jatropha plant. The solubilization index of each isolates was determined on Pikovskaya agar medium. The isolate M (III), M (III) col-2, M (III) col-4, N (b) col-1, N (c) col-2, J(A) and J-C col-2 showed high P solubilization potential having SI=2.11-3.35 recorded and quantitatively solubilized 160, 182, 270, 164, 200, 228 and 182 mg/ml P respectively after 7 day incubation. The isolates were identified and characterized for the plant growth promoting activities such as production of Ammonia, Indole Acetic Acid, Cell wall degrading enzyme; cellulase, chitinase and proteolytic enzyme and antagonistic; against plant pathogenic fungi and bacteria. The P solubilization was accompanied by reduction in pH of the medium. The result indicated the rhizospheric soil is the rich source for isolation of phosphate solubilizing bacteria which promote the plant growth by more than one PGPR trait and have wide application in soil ecology.