Development and characterization of 20 new polymorphic microsatellite markers from Mangifera indica (Anacardiaceae).
Chiang YuChung, Tsai ChiMou, Chen Y. K. H., Lee SheueRu, Chen ChihHsiung, Lin YuShium, Tsai ChiChu
Author Affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
American Journal of Botany 99 : e117-e119
Abstract : Premise of the study: Twenty microsatellite loci for mango (Mangifera indica), an important commercial fruit tree in East Asia, were developed to evaluate the genetic diversity and identification of cultivars. Methods and Results: The 20 new microsatellite markers were isolated from mango using a magnetic bead enrichment method, and polymorphisms were identified in 22 mango cultivars. The number of alleles ranged from one to nine, with expected heterozygosity ranging from 0 to 0.826. The polymorphism information content ranged from 0 to 0.756 with a mean of 0.525. Conclusions: These new microsatellite loci should be useful and convenient for further studies of the genetic diversity and identification of cultivars in mango.