Analysis of genetic diversity among 12 mango cultivars using RAPD markers.
Das B. K., Jena R. C., Samal K. C.
Author Affiliation: Department of Fruit Science, Orissa University of Agriculture and Technology, Bhubaneswar, Orissa, India.
Haryana Journal of Horticultural Sciences 39 : 121-124
Abstract : Randomly Amplified Polymorphic DNA (RAPD) markers were used for the genetic variation and relationship analysis among 12 Mango (Mangifera indica L.) germplasm. PCR amplification with ten RAPD primers generated 150 reproducible and distinct bands, out of which 115 bands (74.22%) were found to be polymorphic. The size of amplified product ranged from 100 to 3000 base pairs. Among all the primers RPI-7 has shown heighest (88.23%) polymorphism while RP1-8 was found to be least polymorphism (50%). Based on Jaccard's Similarity coefficient Arka Punit and Amrapalli cultivar pair was very close to each other with highest similarity coefficient (0.80) On the other hand, Akhurasha vs Mallika and Fazri vs Golag khas cultivar pair was more distinct to each other with the lowest intervarietal similarity coefficient (0.50). This present study clearly showed maximum diversity and indicated the potential of RAPD markers for the identification and management of mango germplasm for breeding purposes.