Preliminary horticultural studies to describe and identify of two new Egyptian mango strains using DNA fingerprint.
Wahdan M. T., Abdelsalam A. Z., El-Naggar A. A., Hussein M. A.
Author Affiliation: Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
The Journal of American Science 7 : 641-650
Abstract : Selection of some strains is considered the first step in improving mango production. So, this study was done for a three successive seasons (2003-2005) on two Egyptian mango strains Hania and Aml to describe them, horticulturally and identification those genetically utilizing DNA fingerprint. The vegetative characters (leaf shape, length, width, etc) and histological characteristics (number of stomata per mm (stomatal density), stomata length and width) showed great variation between the two studied strains. The fruit weight was 581 gm for Hania strain and 1020 gm for Aml strain. The two strains fruits had good characters as shape, net weight ratio, firmness, SSC, TA, Vit. C and total sugars. In generally, physical and chemical properties of Aml strain fruits were better than Hania strain. These differences of horticultural aspects due to genetic variances, which were determined by using SSR markers, of the 42 primers screened, 36 primers gave reproducible polymorphic DNA amplification patterns. 60.7% of the scored fragments are considered putative genotypes-specific markers in both strains. The polymorphic information content (PIC values) ranged from 0.25 to 0.75, with a mean value 0.51 for all loci. The heterozygosity level was 0.68 and 0.53 for Hania and Aml strains, respectively. By banding patterns obtained from these 36 primers, each strain in this study could be distinguished from the other, indicating that, PCR by using SSR primers was an efficient method for genotype identification.