Cloning and characterization of the cDNA gene encoding the light harvesting chlorophyll a/b binding protein in mango.
Luo Cong, Jiang YaQin, Gao MeiPing, Chen Hu, He XinHua
Author Affiliation: College of Agriculture, Guangxi University, Nanning, Guangxi 530004, China.
Journal of Fruit Science 27 : 441-444
Abstract : A full-length cDNA sequence of a homologous Cab gene was cloned by employing RT-PCR, which was named as Mcab. The accession code in GenBank is FJ907952. The Mcab is 915 bp in length, encoding a protein of 264 amino acids, with an estimated molecular weight and an isoelectric point of 28.19 ku and 5.35 respectively. A comparison of the nucleotide sequences of homologous Cab genes from different species indicated that Mcab gene had a range of 72% to 85% identity in nucleotide sequence with homologues of other plants. The results indicated that Mcab protein had one chlorophyll a/b binding domain between the 63st to 232st amino acid and very conservative of the position between woody plants. Protein subcellular localization prediction showed that Mcab protein was located in the chloroplast and the gene was one of important genes in photosynthesis system of green plants. Prediction of the secondary structure of the protein showed that Mcab protein had 8 ? helices, 5 ? sheets and 21 ? turns. The research was beneficial to the further understanding of the molecular mechanism of photosynthesis in mango.