Management of mango (Mangifera indica L.) seedlings obtained in vitro for plant health studies.
López-Estrada M. E., Acosta-Rodríguez M. C., Otero-Colina G., Michel-Aceves A. C., Noriega-Cantú D. H.
Author Affiliation: Brigada de Educación para el Desarrollo Rural No. 90, SEP-DGETA, Maya No. 30, Iguala, Guerrero, C.P. 40000, Mexico.
Revista Mexicana de Fitopatología 26 : 184-187
Abstract : In vitro mango seedlings free of external and seed-transmitted contaminants were obtained from immature embryos. Then, they were transplanted to soil using only sterilized materials. Mango embryos 45-60 days old were plated in modified B5 medium; and after seedlings attained 10 cm height, they were transplanted to soil+expanded perlite in specially designed plastic containers. Plants were irrigated with nutritive universal solution. During the in vitro stage 10% contamination with Penicillium, Aspergillus, Xanthomonas and Cladosporium was observed. Contamination in plastic containers gradually reached 15% after five months. Cladosporium, Alternaria, Aspergillus, Rhizopus, and Fusarium were identified in the substrate, whereas Helminthosporium, Curvularia, Penicillium, and Syncephalastrum appeared in the plastic containers.