A Fusarium sp. different from Fusarium oxysporum and F. mangiferae is associated with mango malformation in Michoacán, Mexico.
Rodríguez-Alvarado G., Fernández-Pavía S. P., Ploetz R. C., Valenzuela-Vázquez M.
Author Affiliation: Laboratorio de Patología Vegetal, IIAF-UMSNH, Km. 9.5 Car. Morelia-Zinapecuaro, Michoacán 58880, Mexico.
Plant Pathology 57 :781
Abstract : In 2004 and 2005, in Michoacán, Mexico, 27 mango trees exhibited typical symptoms of malformation. Isolates were recovered from symptomatic trees, observed for their cultural and morphological characteristics and subjected to molecular analysis. A F. mangiferae-diagnostic PCR primer pair (1-3F/R) did not amplify DNA from the Michoacán strains, but a primer pair that amplifies DNA from F. sterilihyphosum but not F. mangiferae (61-2F/R) amplified the reported 445-bp fragment. The Michoacán strains grew more than 50% faster than F. sterilihyphosum, and possessed a 625-bp sequence of the translation elongation factor-1? that differed at 18 nucleotide positions from a F. sterilihyphosum sequence in GenBank (Accession No. DQ4528581) of which were found in the affected trees. A taxon other than F. mangiferae causes mango malformation in Michoacán. Our findings challenge previous reports that F. oxysporum causes mango malformation in Michoacán, though isolates from mango were not available.