Physiologic and pathogenic characterization, and isozyme analysis of monosporic and multisporic isolates of Colletotrichum gloeosporioides.
Serra I. M. R. de S., Coelho R. S. B., Menezes M.
Author Affiliation: Universidade Federal Rural de Pernambuco, UFRPE, Departamento de Agronomia/Fitossanidade, CEP 52171-900, Dois Irmãos, Recife, PE, Brazil.
Summa Phytopathologica 34 : 113-120
Abstract : Monosporic isolates of Colletotrichum gloeosporioides [Glomerella cingulata] obtained from 6 cashew and mango trees and multisporic isolates were characterized for colony morphology, mycelial growth on BDA, pathogenicity and isoenzyme analysis. The colonies of multisporic isolates presented diversity of colour and aspect, but expressive morphological variations were not observed in 60 monosporic isolates compared to their respective multisporic isolates. Different morphological characteristics were observed only in 2 isolates. All the studied isolates showed good growth on BDA until 6 days after incubation. Some monosporic isolates obtained from 4 cashew and mango and multisporic ones showed heterogeneous mycelial growth. Pathogenicity was the variable that best characterized the variation between monosporic and multisporic isolates of C. gloeosporioides. Different aggressiveness levels were observed among and within monosporic and multisporic isolates. In the analysis of isoenzymes, the electrophoretical patterns of isoesterases showed no variation among the monosporic isolates and the parental multisporic ones. The results suggested that the selection of the C. gloeosporioides type isolate for studies on cashew and mango should be preceded by a comparison between monosporic and multisporic isolates based upon mycelial growth and pathogenicity.