Isolation and characterization of mRNAs differentially expressed during ripening of mango fruits.
Saiprasad G. V. S., Lalitha Anand, Ravishankar K. V., Mythili J. B., Nagesh M., Joshi R.
Author Affiliation: Molecular Biology Laboratory, Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore 560 089, India.
Indian Journal of Biotechnology 3 : 533-537
Abstract : Mango, an important climacteric fruit crop with low shelf life, requires technologies to increase shelf life to reduce post harvest losses. During ripening of mango, the authors isolated five ripening related cDNAs from two mango cultivars, Alphonso and Totapuri, using RT-PCR technique. The predicted polypeptides of five of these clones exhibit similarity to database protein sequences of PRL-1 protein, transcription initiation factor, CCR-4 protein, 18S ribosomal RNA gene and 23S ribosomal RNA gene. None of these proteins appear to be directly related to events generally associated with ripening such as cell wall metabolism or the accumulation of sugars and pigments or ethylene biosynthetic pathway. They are the regulatory elements/signals known to be involved during fruit ripening and may, therefore, be involved in regulating the expression of other genes directly associated with fruit ripening. The probable role of these proteins in mango fruit ripening needs to be elucidated further.