A short note on Micro-anatomical studies of graft joints of stone grafted mango (Mangifera Indica L.) var. Amrapali.
Pandey V., Singh J. N.
Author Affiliation: Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi - 221 005, U.P., India.
Orissa Journal of Horticulture 31 : 123-126
Abstract : In studies conducted in Uttar Pradesh, India, during 1995-96, micro-anatomical study of graft joint was taken up to observe the status of graft union between stock and scion tissues of successfully sprouted mango stone grafts of Amrapali cultivar both in healthy and in partially wilted plants at 2- and 4- month after grafting. Graft joints were collected, preserved in hydrofluoric acid solution, transferred to 70, 80, 90, 95 and 100% ethyl alcohol and kept for 72, 48, 24, 12 and 6 h, respectively, and specimens were embedded and stained. Even on successfully sprouted and healthy grafts, the union between rootstock and scion was incompletely marked by a light brownish band. However, in the case of partially wilted grafts, a deep dark brown to black band of tissues was found at 2 months after grafting. At 4 months after grafting, however, the union between rootstock and scion in healthy grafts was complete. However, in partially wilted grafts, the complete union between rootstock and scion failed to take place, marked by prominent deep brownish-black band of dead tissues. The results indicated that initial sprouting and survival of stone-grafted plants was basically due to callus (parenchymatous) tissues, which allowed partial translocation of vital biochemical compounds between rootstock and scion.