Estimation of genetic diversity of commercial mango (Mangifera indica L.) cultivars using RAPD markers.
Kumar N. V. H., Narayanaswamy P., Prasad D. T., Mukunda G. K., Sondur S. N.
Author Affiliation: Division of Horticulture, UAS, GKVK, Bangalore, India.
Journal of Horticultural Science and Biotechnology 76 : 529-533
Abstract : Fifty mango cultivars, chosen from a number of southern Indian institutes to represent a wide range of geographical sources, were screened using randomly amplified polymorphic DNA (RAPD) markers with decamer primers of arbitrary sequence. Out of the 80 primers screened, ten were selected which gave 139 clear and bright fragments. A dendrogram based on Jaccard's co-efficient of similarity implied a moderate degree of genetic diversity among the cultivars used for experimentation. The hybrids which had one parent in common were placed together. In the cluster, alternate bearers and regular bearers formed separate groups, and the members in each group were very closely linked. Another analysis based on Pearson's co-efficient of similarity revealed a high degree of genetic diversity. In both the analyses, Mulgoa was found to be very distinct. This study showed clearly that cultivars from South India possess a high degree of genetic diversity and indicated the potential of RAPD markers for the identification and management of mango germplasm for breeding purposes.