A rapid method for differentiation of Xanthomonas campestris pv. mangiferaeindicae from other Xanthomonads and mango phylloplane inhabitants.
Sanders G. M., Korsten L.
Author Affiliation: Department of Microbiology and Plant Pathology, New Agricultural Building, Faculty of Biological and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa.
Journal of Basic Microbiology 35 : 337-347
Abstract : Several selective media were evaluated for selective enrichment of X. c. pv. mangiferaeindicae from mango plants. Boost broth supplemented with cycloheximide, methyl violet and methyl green enhanced growth of epiphytic X. c. pv. mangiferaeindicae but not other phylloplane organisms. The identity of X. c. pv. mangiferaeindicae selectively enriched from mango plant material was confirmed with monoclonal antibodies in ELISA. Preliminary field evaluations showed that selective enrichment and confirmation by ELISA could successfully detect latent infections of X. c. pv. mangiferaeindicae in mango plants.