Rapid molecular detection of Fusarium mangiferae, the pathogen of mango malformation disease.
Wu JingBo, Zhan RuLin, Liu Feng, Zhao YanLong, He YanBiao, Chang JinMei
Author Affiliation: South Asia Tropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, Guangdong 524091, China.
Mycosystema 35 : 298-308
Abstract : Mango malformation disease (MMD), caused by Fusarium, is one of the most destructive diseases on mango, imposing continuous threat on future mango industries. The disease is difficult to diagnose and short of effective control methods. Based on ISSR molecular marker, a purpose primer (UBC 888) was selected from 50 primers, and a stable and specific band of 479 bp (GenBank Accession KJ526382) was generated. According to the specific sequence, the authors designed a pair of SCAR primers (W342, W1772) and got a specific gene fragment of 1 376 bp (GenBank Accession KJ526383), and the ISSR molecular markers were successfully transformed to SCAR ones. An effective detection technique of mango malformation disease was established based on this pair of primers. The established technique is simple, of high specificity and sensitivity to the pathogen both in vitro and in vivo, with the lowest detecting limit of 10 pg fungal DNA. This assay provided a theoretical basis and technical method for early diagnosis and proper prevention of mango malformation disease.