Biodiversity of six varieties of Mangifera indica using RAPD.
Gurijala H. K., Rampa D. R., Jasti P. K.
Author Affiliation: Department of Biotechnology, Sri Venkateswara University, Tirupati, AP, India.
International Journal of Life Sciences Biotechnology and Pharma Research 4 : 100-103
Abstract : The objective of our study was to identify the genetical diversity of six populations of Mangifera indica using Random Amplified Polymorphic DNA (RAPD) markers. Genomic DNA was extracted, subjected to PCR with the primers OPAB01, OPAB 04, OPAB 05, OPAB 06, OPAB 08, OPAB 09, OPAB 12, OPAB 14, OPAB 15, OPAB 19, OPAB 20, A2, A3, A 10, A 18, P10 and W18. Out of 17 RAPD primer tested, 15 showed polymorphism in the six populations of Mangifera. The means of each population contained in average 16.58 was found. From 15 primers 277 bands were found, out of them 94 bands shown polymorphism at the rate of 31.23%. Phylogenetic variability among all plants samples evaluated that Thothapuri was the most prominent resembling species with all of the remaining species following Malgoba, Rasapuri, Neelam, Badhami and Sendura.