Urea induced urease activity is coupled to the synthesis of primary monoamine: a possible mechanism of enhancing fertilization of mango flower.
Haque M. S., Roy U. K., Neela F. A., Islam M. M., Kabir M. F.
Author Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Protein and Enzyme Research, University of Rajshahi, Rajshahi-6205, Bangladesh.
International Journal of Agricultural Technology 10 : 1545-1556
Abstract : Mango is a delicious and nutritious fruit however its production is retarded by different ways although the mechanism is not clarified. In this study, we analyzed mango flower and examined the effect of urea on urease activity in flower extract. The flower had 12.0 g of protein, 1.21 g of lipid, 2.65 g of sugar, 0.01 g of reducing sugar, 4.01 g of starch, non reducing sugar 2.64 g in 100 g of flower and the moisture content is 77.15%. The higher urease activity in response to 100 mM urea was found in flower extract rather than 50 and 200 mM concentrations. The Km and Vmax were 62.5 mM and 0.04 µmole/mg of protein/min for the above doses respectively. Moreover, 100 mM urea was found to cause a higher synthesis of protein in the flower. Primary monoamine has been recognized to be the fertilization of flower and was found to increase whenever the flower extract was treated with 100 mM urea. Therefore, the results demonstrate that urea is involved in enhancing urease activity which is coupled to the synthesis of protein and the overall bioprocess might be linked to the higher synthesis of primary amine augmenting the higher fertilization of mango flower.