Pathogen identification and pathogenicity analysis of grape ripe rot in Fujian.
Lei Yan, Lin XiongJie, Chen Ting, Liu XinMing, Cai ShengHua, Fan GuoCheng
Author Affiliation: Fruit Research Institute, Fujian Academy of Agriculture Science, Fuzhou, Fujian 350013, China.
Journal of Fruit Science 31 : 1123-1127
Abstract : Objective: To know the pathogen species, pathogenicity and host range of grape ripe rot in the main production area of Fujian Province. Method: Morphological methods combined with molecular biology were used to isolate and identify the strains of grape ripe rot in this study. Result: Two strains of Colletotrichum gloeosporioides were isolated according to colony morphology. The conidia were single cell, colorless, and most of them were cylindrical, rod-shaped or oval. The appressorium was dark brown, and most of them were spherical or nearly spherical. One band with about 500 bp could be amplified from the genomic DNA of all the tested strains with C. gloeosporioides specific primers CgInt/ITS4. They could infect apple, mango, nectarine, plum, pear, tomatoes and other fruits in the host range test. Conclusion: The results suggested that the pathogen of grape ripe rot was C. gloeosporioides in the main grape production area of Fujian province. The isolates could infect 'Red Globe' grape, apple, mango, pear and other fruits, and they had a wide range of host.