Molecular modeling and expression analysis of a MADS-box cDNA from mango (Mangifera indica L.).
Pacheco-Sánchez M. A., Contreras-Vergara C. A., Hernandez-Navarro E., Yepiz-Plascencia G., Martínez-Téllez M. A., Casas-Flores S., Arvizu-Flores A. A., Islas-Osuna M. A.
Author Affiliation: Plant Molecular Biology Lab, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Apartado Postal 1735, 83304 Hermosillo, Sonora, Mexico.
3 Biotech 4 : 357-365
Abstract : MADS-box genes are a large family of transcription factors initially discovered for their role during development of flowers and fruits. The MADS-box transcription factors from animals have been studied by X-ray protein crystallography but those from plants remain to be studied. In this work, a MADS-box cDNA from mango encoding a protein of 254 residues was obtained and compared. Based on phylogenetic analysis, it is proposed that the MADS-box transcription factor expressed in mango fruit (MiMADS1) belongs to the SEP clade of MADS-box proteins. MiMADS1 mRNA steady-state levels did not changed during mango fruit development and were up-regulated, when mango fruits reached physiological maturity as assessed by qRT-PCR. Thus, MiMADS1 could have a role during development and ripening of this fruit. The theoretical structural model of MiMADS1 showed the DNA-binding domain folding bound to a double-stranded DNA. Therefore, MiMADS1 is an interesting model for understanding DNA-binding for transcriptional regulation.