Molecular characterization of traditional mango (Mangifera indica L.) varieties of Kerala.
Simi S., Rajmohan K., Soni K. B.
Author Affiliation: Department of Plant Biotechnology, College of Agriculture, Kerala Agricultural University, Thiruvananthapuram (Kerala), India.
Asian Journal of Horticulture 8 : 323-327
Abstract : Investigations were done with the objective to characterize the traditional mango varieties/accessions of Kerala based on Randomly Amplified Polymorphic DNA (RAPD) markers. Thirty accessions/ecotypes were subjected to RAPD analysis. DNA was extracted from young leaves using CTAB method with slight modification. A total of 157 RAPDs (average of 3.74 bands per primer) were generated on PCR amplification using 42 decamer primers, of which 96.18 per cent (151 bands) were polymorphic. Of these, ten primers yielded 92 scorable bands with an average of 9.2 bands per primer. The pair wise similarity coefficient values ranged from 0.217 to 0.833. In the dendrogram, the thirty accessions were observed to group into seven clusters. The largest cluster contained 20 accessions. Four table varieties, Kappa manga, Mylapore manga, NeendaKarpooram and Kandiyoor Local were grouped together. All these were soft fleshed. Muthalamookan and Kolambi manga clustered together. Puliyan, Perakka manga, Kalluketty and ChampaVarikka formed four separate clusters. These ecotypes with desirable traits can be used to develop molecular markers so that a particular gene of interest can be identified and transferred to a desirable cultivar through genetic engineering.